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P7 - Deciphering sialic acid O-acetylation

Sialic acids (Sias) show a pronounced structural diversity that arises from post-synthetic modifications of a shared nine-carbon sugar backbone. The Mühlenhoff lab has identified CASD1 as key enzyme in the biosynthesis of 9-O-acetylated sialoglycans and setup a unique O-acetylation specific toolbox. In preliminary work, we demonstrated that genetic ablation of CASD1 in mice results in a complete loss of 9-O- and 7,9-O-acetylated sialoglycans and causes a kidney phenotype. To understand how sialic acid O-acetylation contributes to kidney integrity, we will perform functional assays on isolated primary cells and explore the contribution of O-acetylated sialoglycans on tubular epithelial and vascular endothelial cells through the generation of cell type-specific Casd1 knockout mice. In parallel, we will pursue studies to unravel the interplay of CASD1, sialyltransferases and the catabolic sialate 9-O-acetylesterase SIAE in shaping the Sia O-acetylome. Within the research unit, our project is closely linked to projects 2, 5 and 9, and will benefit from specific interactions with projects 1, 6, and 8.

PD Dr. Martina Mühlenhoff

PD Dr. Martina Mühlenhoff

Principal Investigator

Hannover Medical School

Institute of Clinical Biochemistry

Carl-Neuberg-Strasse 1

30625 Hannover

Tel.: +49 511 532-6547

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